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The c.-582A > G variant decreases hepcidin promoter transcription activity. HepG2 cells were transfected with VarA-Hep/Luc or VarG-Hep/Luc constructs alone (basal) and cotransfected with pCMV-USF1, pCMV-USF2 or pCMV-USF1+ pCMV-USF2 (1:1). Data represent means of three independent experiments (± SEM) ***p < 0.001, *p < 0.05
