Figure 1.

Procedure for the reconstruction of genome-scale metabolic network in Z. mobilis and its application to metabolic engineering. Automatic reconstruction of metabolic network based on genome sequence and annotation data (1-2-3-4). Manual curation of the metabolic network using literatures, databases, gene-reaction correlation, and comparative genomics to correct errors and fill gaps in the pathways (4-7-8-2 or 5-6-8-2). Determination of biomass composition and validation of metabolic model in comparison with experimental data (9-10-11). Systems metabolic engineering for strain improvement by combining experimental and in silico approaches (12-13). The detailed information about fermentation profile (11), utilized to refine reconstructed metabolic model is in additional file 7.