Figure 2.

Central metabolism and analysis of flux-sum intensity in Z. mobilis. (A) Central metabolism, including ED Pathway and TCA cycle in Z. mobilis based on annotated genes. Reactions for utilizing pentose sugars in Z. mobilis are introduced and represented to blue lines. The red lines indicate the missing reactions in Z. mobilis. (B) Flux-sum intensity of selected metabolites, including cofactors, amino acids and others, in central metabolism for Z. mobilis and E. coli, respectively, for three carbon sources (i.e. glucose, fructose, and sucrose). Uptake rate of each carbon source is fixed to 10 mmol/gDCW/h, and reaction for oxygen uptaking was deleted to describe anaerobic condition in both organisms. NGAME (i.e. non-growth associated maintenance energy) value for both organisms was eliminated. Upper constraints (i.e. limit the flux on fumaric acid, acetic acid, malic acid, acetoin, and acetaldehyde production) in Z. mobilis metabolic model for regulating the flux more realistically are relieved. Results are normalized by maximum value of each metabolite. Darker color indicates stronger flux-sum intensity.