Figure 3.

Characteristics of aerobic shift and strategies for succinic acid production in Z. mobilis. (A) Trade-off curves of Z. mobilis for the flux rate of NADH oxidase reaction, ethanol production rate, and growth rate under aerobic condition. The relationship between growth rate and ethanol production rate was investigated for several flux levels of NADH oxidase reaction constrained by 0, 1, 2, and 2.8 mmol/gDCW/h under aerobic condition. The vertical lines of each graph that meet x-axis drop to zero. (B) Trade-off curves of wild type and mutant (Δpdc and ΔldhA) strains of Z. mobilis for succinic acid production under anaerobic condition. The black solid line and the red dotted line indicate the flux solution space of the wild type and mutant strain of Z. mobilis, respectively. Each circle denotes the state of the wild type and mutant strain of Z. mobilis on each maximal growth rate, respectively.