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. 2010 Nov 26;9:95. doi: 10.1186/1475-2859-9-95

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Copyright ©2010 Gallo et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gel band shift assays. EMSA was carried out using DNA fragments upstream A. balhimycina ppk (Pppk Amy) and pstS (PpstS Amy) and a crude extract from A. balhimycina LP culture. The use of a molar excess (200 fold) of unlabeled probe as well as of a DNA fragment upstream S. coelicolor pstS (PpstS Sco), containing a PHO box, reduced the band shift signals for both probes. No reduction of band shift signals is visible using a molar excess (200 fold) of an unspecific competitor (Nonomuraea Pdbv14 [14]).