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. 2010 Dec 6;9:98. doi: 10.1186/1475-2859-9-98

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Copyright ©2010 Hartwig et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Antigenicity of the various forms of rLigANI and rLipL32. Antigenicity was evaluated using rabbit anti-Leptospira sera (A) Lanes: 1 - rLigANI + PNGase F; 2 - rLigANI + Endo H; 3 - glycosylated rLigANI and (B) Lanes: 4 - rLipL32 + PNGase F; 5 - rLipL32 + Endo H; 6 - glycosylated rLipL32 or (C) convalescent sera from leptospirosis patients, Lanes: 1 - glycosylated rLipL32; 2 - glycosylated rLigANI and (D) 3 - rLipL32 + PNGAse F; 4 - LipL32 + Endo H; 5 - rLigANI + PNGAse F; 6 - rLigANI + Endo H. E-LigANI (61 kDa) and E-LipL32 (32 kDa) recombinant proteins were expressed and purified from E. coli.