Table 3. IC₅₀ and dissociation constant (K_d) for the inhibition of thrombin by various variants of CrSPI-1-D1.

S.No.	Mutants	IC50	Kd (dissociation Const.)
1	T4A	ND	ND
2	Y5K	ND	ND
3	K6H	ND	ND
4	P7R	ND	ND
5	T4A,Y5K	ND	ND
6	T4A,K6H	ND	ND
7	T4A,P7R	ND	ND
8	Y5K,K6H	ND	ND
9	Y5K,P7R	ND	ND
10	K6H,P7R	ND	ND
11	T4A,Y5K,K6H	ND	ND
12	T4A,Y5K,P7R	ND	ND
13	T4A,K6H,P7R	ND	ND
14	Y5K,K6H,P7R	ND	ND
15	T4A,Y5K,K6H,P7R	26.3 nM	4 µM

ND- not detected.