Figure 2. Secretion profile of growth factors and cytokines from immortalized hMSCs.

Immortalized hMSCs were cultured in DMEM/LG medium for 48 hours. The cultured media were collected and applied onto a human growth factors/cytokines antibody array membrane (left panel in A). The map of the array that is designed to detect 60 cytokines, chemokines, or growth factors is shown in A (right panel). The arrays were scanned, and the staining intensity of each spot was quantified by densitometry. The percentage of the intensity of the respective spots over that of the positive control (POS) on the same array was represented as the relative expression levels of each human growth factor (B) or cytokine (C). The data represent mean ± SEM of three independent experiments.