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. 2010 Nov 24;8:8. doi: 10.1186/1476-8518-8-8

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Copyright ©2010 Han et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expansion of CD34+ HPCs and phenotype analysis. (A) Ex vivo expansion kinetics of CD34+ HPCs in LSC culture. HPCs were purified using anti-CD34 Ab magnetic beads and the expansion kinetics on LSC-KFT63b of HPCs from four donors are plotted at bottom. The purified CD34+ cells were analyzed with anti-CD34, anti-CD133, and anti-CD33 Abs by flow cytometry and representative flow graphs of mobilized PB CD34+ HPCs from three donors are illustrated. (B) Flow cytometry analysis of hematopoietic progenitor and differentiation markers after HPC expansion in LSC culture for 9 days. The CD34+ HPCs of cord blood and adult PB were analyzed and presented.