Table 1.
Caco-2 cell genes involved in intracellular junction complex formation that were differentially expressed in the microarray analysis after co-culturing with L. plantarum MB452 (OD600 nm 0.9) for 10 hours.
| Gene Name | Symbol | Refseq ID | Fold Change | Moderated | Description of role in relation to tight junctions |
|---|---|---|---|---|---|
| occludin | OCLN | NM_002538 | 1.39 | 0.004 | tight junction bridging protein |
| vascular endothelial growth factor A | VEGFA | NM_001025366 | 1.39 | 0.002 | cytokine that indirectly regulates tight junction formation and strengthening |
| actin beta | ACTB | NM_001101 | 1.33 | 0.005 | structural constituent of cytoskeleton |
| cingulin | CGN | NM_020770 | 1.29 | 0.024 | tight junction plaque protein associated with occludin |
| par-6 partitioning defective 6 homolog beta | PARD6B | NM_032521 | 1.27 | 0.009 | tight junction plaque protein associated with claudins and involved in cell polarization |
| actin alpha cardiac muscle 1 | ACTC1 | NM_005159 | 1.25 | 0.015 | structural constituent of cytoskeleton |
| itchy homolog E3 ubiquitin protein ligase | ITCH | NM_031483 | 1.25 | 0.011 | ubiquitin-ligase molecule that regulates occludin degradation |
| junction plakoglobin | JUP | NM_002230 | 1.24 | 0.010 | major cytoplasmic protein that forms a complex with cadherins |
| CNKSR family member 3 | CNKSR3 | NM_173515 | 1.24 | 0.006 | tight junction plaque protein associated with JAMs |
| snail homolog 1 | SNAI1 | NM_005985 | 1.24 | 0.033 | intracellular component that indirectly inhibits occuldin production |
| hepatocyte nuclear factor 4 alpha | HNF4A | NM_178849 | 1.24 | 0.021 | transcription regulator that acts on occuldin |
| zona occludens 1 (tight junction protein 1) | ZO-1 | NM_003257 | 1.23 | 0.013 | tight junction plaque protein associated with occludin, JAMs and claudins |
| zona occludens 2 (tight junction protein 2) | ZO-2 | NM_004817 | 1.23 | 0.054 | tight junction plaque protein associated with occludin and claudins that acts as a guanylate kinase and also found in the nucleus |
| CD2-associated protein | CD2AP | NM_012120 | 1.22 | 0.012 | scaffolding molecule that regulates the actin cytoskeleton |
| vinculin | VCL | NM_003373 | 1.22 | 0.027 | cytoskeletal protein |
| membrane associated guanylate kinase 3 | MAGI-3 | NM_152900 | 1.21 | 0.044 | tight junction plaque protein a and guanylate kinase |
| membrane protein, palmitoylated 5 | MPP5 | NM_022474 | 1.20 | 0.014 | tight junction plaque protein associated with claudins and guanylate kinase involved in tight junction organization |
| cleavage and polyadenylation specific factor 2 | CPSF2 | NM_017437 | -1.22 | 0.022 | transcription regulator that decreases tight junction stability |
| cyclin-dependent kinase 4 | CDK4 | NM_000075 | -1.30 | 0.011 | transcription regulator that decreases tight junction stability |