Table 1.
Primer | Sequence | Purpose |
---|---|---|
R1 | GTAGTCCGGGTGATCCTTCTTGTGC | RACE |
R2 | AGACGCTCAGCCTCCTCCACGAA | RACE |
R3 | AGCCTGAGCCCACACCATGAACG | RACE |
F1 | TCAGCGTCAGCCCAGGTCAGGAGA | RACE |
F2 | TGTTTCAGGGCTGTGGAGTTGGTGG | RACE |
F3 | GCAAACGCAAACTGGCAGAGACGC | RACE |
UTR-F | CGGTCTCACAATGGGAACCAACTC | Full-length cDNA amplification and tissue distribution |
UTR-R | GCTGAACATCTCCTAGAGCAGTCCA | Full-length cDNA amplification and tissue distribution |
β-actinF | GGCATCACACCTTCTACAACGA | Internal control |
β-actinR | ACGCTCTGTCAGGATCTTCA | Internal control |