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. 2010 Dec 20;5(12):e15678. doi: 10.1371/journal.pone.0015678

Figure 3. Effect of DNAse I and DNA on biofilm mass.

Figure 3

A) The lysogenic strain R36AP and its non-lysogenic parent R36A were exposed from seeding to DNAse I at final concentrations of 0.5, 5 and 50 µg/ml. Biofilm mass was quantified after 24 h of incubation. B) R36AP and R36A were exposed from seeding to DNA from R36A or salmon sperm at final concentrations of 10, 100 and 1000 ng/ml. In separate experiments, the effect on biofilm development of coating the wells with 1000 ng/ml of R36A DNA prior to seeding and the addition of sonicated R36A DNA at 1000 ng/ml since the time of seeding was also determined. Biofilm mass was assessed at 24 h of incubation. In all panels, the results are an average of 9 independent replicates and error bars represent 95% confidence intervals for the sample mean.