Skip to main content
. 2010 Dec 20;5(12):e15678. doi: 10.1371/journal.pone.0015678

Figure 4. eDNA quantification and DNA impact on biofilm mass.

Figure 4

A) Extracellular DNA was isolated from the biofilm matrices of R36A, R36AΔlytA, R36AP, R36APΔsvl, R36APΔlytA and R36APΔlytAΔsvl and quantitative real-time PCR of two chromosomal genes, spi and gdh, was done. The relative biomass was quantified at OD595 nm and the eDNA measurements were normalized to total biofilm mass. B) The effect of salmon sperm DNA (1000 ng/ml) added from seeding on biofilm biomass at 24 h was tested. R36APΔsvl, R36APΔlytA, R36AΔlytA and R36APΔlytAΔsvl are mutants in which the phage lysin (Svl), the bacterial autolysin (LytA) or both were deleted. C) The same experiments described in panel B were done with the encapsulated wild type host of phage SV1, strain SVMC28, and its mutants. SVMC28Δsvl, SVMC28ΔlytA and SVMC28ΔlytAΔsvl are mutants in which the phage lysin (Svl), the bacterial autolysin (LytA) or both were deleted. In all panels, the results are an average of 9 independent replicates and error bars represent 95% confidence intervals for the sample mean.