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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 Nov 3;175(2):192–195. doi: 10.1016/j.molbiopara.2010.10.009

Figure 2. Mapping of the NLS for TgGCN5-B.

Figure 2

IFAs using antibody to FLAG tag were used to detect various forms of TgGCN5-B or β-gal fusion proteins. Diagram of each protein is shown to the right with FLAG epitope tag and proteins domains indicated. The blue box of each TgGCN5-B protein diagram represents the HAT catalytic domain and the orange box depicts the bromodomain. β-gal protein cartoon is represented in green. A. Localization of TgGCN5-B lacking the first 313 (Δ313) or B. 310 (Δ310) amino acid residues. C. Localization of TgGCN5-B after an internal deletion of the ten residue NLS (ΔNLS). D. Localization of β-galFLAG. E. Localization of β-gal-NLSFLAG. F. Localization of GCN5-BΔ310 containing alanine substitutions for R311 and P312. hN, host cell nucleus; TgN, Toxoplasma nucleus; green = anti-FLAG; red = DAPI.