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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Oct 15;43(1):120–129. doi: 10.1016/j.biocel.2010.10.003

Fig. 4.

Fig. 4

Knockdown of Plk3 expression results in multinucleated cells. (A) Immunoprecipitate of T47D cell lysates using anti-CIB1 or cIgG1 and immunoblotted for Plk2, Plk3, and CIB1 as indicated. (B) Immunoblots of cells transfected with pSuper or Plk3-shRNA construct probed with anti-Plk1 or anti-Plk2 or anti-Plk3 or anti-CIB1 as indicated. (C) Light microscopic images of cells from B. Bar: 10 µm. (D) Quantitation of the number of multinucleated cells as a function of Plk3 shRNA concentration (*P<0.01).