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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Pharmacol Ther. 2010 Oct 31;129(1):21–28. doi: 10.1016/j.pharmthera.2010.09.012

Fig. 2. Representative images of human peripheral blood- derived VSEL and HSPC by ImageStreamX system.

Fig. 2

Human blood cells were stained for markers distinguishing VSELs such as: i) CD45 panleukocytic antigen (APC-Cy7, cyan), ii) hematopoietic lineages markers (FITC, green) and iii) stem cell antigens CD133 (PE, yellow) and CD34 (APC, violet). Nuclei were stained with Hoechst 33342 dye. Images were collected by imaging flow cytometer – ImageStreamX system. VSELs and HSPCs were distinguished based on CD45 antigen expression.