Table.

Major differences between isolation of murine bone marrow-derived and human umbilical cord blood-derived cells.

	Murine BM-VSELs	Human VSELs
SURFACE MARKERS	Sca-1+, CXCR4+, CD133+, CD34+, SSEA-1+, AP+, c-Met+, LIF-R+, CD45−, Lin−, HLA-DR−, MHC I −, CD90−, CD29−, CD105−	CXCR4+, CD133+, CD34+, SSEA-4+, AP+, c-Met+, LIF-R+, CD45−, Lin−, HLA-DR−, MHC I −, CD90−, CD29−, CD105−
SIZE	4–5 µm	6–8 µm
FREQUENCY	0.03% of nucleated cells	0.01% of nucleated cells
TISSUES	Bone marrow, peripheral blond, brain, muscle, thymus, heart, liver, kidney, testes, spleen, lung, retina, pancreas, fetal liver	Bone marrow, umbilical cord blood, peripheral blood
ANTIBODIES USED FOR FACS-BASED ISOLATION	anti mouse-Ly-6/E (Sca-1), anti mouse-CD45, anti mouse-lineage: CD45R/B220, Gr-1, TCRαβ, TCRγδ, CD11b, Ter119	anti-human-CD133, anti-human-CD45, anti-human-lineage: CD2,CD3, CD14, CD66b, CD24, CD56, CD16, CD19, CD235a