Figure 5. γH2AX foci after irradiation of GBMJ1 cells: in vivo (ic) vs. in vitro.
Intracerebral xenografts initiated from CD133+ GBMJ1 cells were locally irradiated (6Gy) at the initial signs of morbidity (72–94 days after implantation). (A) γH2AX foci induction and dispersal after irradiation of ic xenografts. Values shown represent the mean ± SD of 3–4 mice in which 50 tumor cells were scored per mouse. (B) monolayer cultures of CD133+ GBMJ1 cells grown in stem-cell media (black bars) or 10% FBS (gray bars) were irradiated (2 Gy) and analyzed at the specified times. Values shown represent the mean ± SD of 3 independent experiments in which 50 cells were scored per time point. * p < 0.05 as compared to unirradiated control