Fig. 3.

Crystals of PSII core complex. a Typical morphology of crystals in the crystallization drops. b Diffraction pattern under cryogenic conditions with a limiting resolution of 7.0–7.8 Å. c SDS-PAGE analysis (Coomassie staining) of the protein content of the crystals. Crystals were harvested from a crystallization drop, washed extensively and dissolved in loading buffer. Lane 1 was loaded with molecular marker, lane 2 with washing buffer and lane 3 with the solution containing the dissolved crystals. The complex was composed of the subunits CP47, CP43, PsbO, D1, D2 and PsbE. The subunit identification was based on the analyses of Barber et al. (1997) and Fey et al. (2008)