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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Res. 2010 Oct 20;70(24):10351–10361. doi: 10.1158/0008-5472.CAN-10-0740

Figure 5. N-Myc and HIF-1α contribute to the Warburg effect of MYCN amplified neuroblastoma cells.

Figure 5

(A) MYCN amplified neuroblastoma cells exhibit the Warburg phenotype. Glucose consumption and lactate production in LAN5, Kelly, NLF, SK-N-DZ and IMR32 cells cultured at normoxia for 24 hr. Data were averaged from triplicates.

(B) N-Myc regulates expression of specific genes involved in glycolysis. N-Myc was inhibited by control or specific shRNAs in LAN5 cells. Expression of glycolytic genes was determined by QRT-PCR and shown as an average of triplicates. *p< 0.001.

(C) HIF-1α regulates the expression of specific glycolytic genes at normoxia. Expression of glycolytic genes in control and HIF-1α knockdown cells was determined by QRT-PCR and shown as an average of triplicates. *p< 0.005.

(D) Effects of simultaneous HIF-1α and N-Myc inhibition on HK2, PDK1 and LDHA expression. N-Myc and HIF-1α were depleted by control or specific shRNAs in LAN5 cells. Expression of HK2, PDK1 and LDHA was determined by QRT-PCR and shown as an average of triplicates. *p< 0.01.