(A) Relative expression of LDHA in primary neuroblastoma tumors. LDHA levels in fetal brain were used as a control. Tumor numbers: low risk group (28), intermediate (21), MYCN single-copy, high risk (32) and MYCN amplified, high risk (20).
(B) Depletion of LDHA expression profoundly inhibits the proliferation of MYCN amplified neuroblastoma cells. The LDHA protein is indicated by arrowhead. *p< 0.01.
(C) Depletion of LDHA expression inhibits the tumorigenic capacity of Kelly cells (8 tumors were analyzed in each group).
(D) A model depicting N-Myc and HIF-1α cooperation in neuroblastoma tumor progression. See text for details.