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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 6;386(1):101–104. doi: 10.1016/j.bbrc.2009.05.132

Fig. 1.

Fig. 1

Unglycosylated gp130 in NECs treated with tunicamycin. NECs were treated with tunicamycin for 8 h, stimulated with LIF (0 or 40 ng/ml) for 10 min and then lyzed. Lysates and immunoprecipitated gp130 were subjected to Western-blot analysis. NEC lysates were analyzed with anti-HNK-1 antibody, anti-β-actin antibody and anti-gp130 antibody. Anti-HNK-1 antibody was used to detect glycoproteins as a control. Anti-β-actin antibody was used to confirm that the same amount of proteins was applied to each lane. Immunoprecipitated gp130 was analyzed with anti-phospho-tyrosine antibody (4G10) to evaluate the activation. The molecular masses are indicated on the right of each panel.