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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 6;386(1):101–104. doi: 10.1016/j.bbrc.2009.05.132

Fig. 2.

Fig. 2

Cell surface localization of unglycosylated gp130 in NECs treated with tunicamycin. To confirm the cell surface localization of unglycosylated gp130, cell surface proteins expressed in NECs treated with or without tunicamycin (2 μg/ml) for 8 h were biotinylated by treating the cells with sulfo-NHS-LC-biotin. After lysis, biotinylated cell surface proteins were pulled down with streptavidin-conjugated agarose and then analyzed by Western-blot using anti-gp130 antibody (A) or anti-LIFR antibody (B).