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. Author manuscript; available in PMC: 2011 Aug 11.
Published in final edited form as: Structure. 2010 Aug 11;18(8):955–965. doi: 10.1016/j.str.2010.04.017

Figure 4. Interaction of E4B U-box with UbcH5c and Ubc4 E2 Conjugating Enzymes.

Figure 4

(A) Isothermal titration calorimetry of UbcH5c with E4B U-box. Shown are the integrated heat measurements from injecting 3 mM E4B U-box into the calorimeter cell containing UbcH5c at an initial concentration of 100 μM (top panel) or buffer solution (middle panel). A standard one-site model was used for curve fitting (bottom panel) in the determination of KD and stoichiometry (n), the values of which are shown.

(B) Crystal structure of human E4B U-box in complex with UbcH5c.

(C) Crystal structure of mouse CHIP U-box in complex with Ubc13 (PDB entry 2C2V).

(D) Crystal structure of the human c-Cbl RING E3 ligase in complex with UbcH7 (PDB entry 1FBV).

See also Figure S2.