Table 2.

Crystallography Statistics

	E4B U-box	Ubc4	E4B U-box–UbcH5c
Data collection
Space group	P 4 3 2	P 1 21 1	P 6 2 2
Cell dimensions
a, b, c (Å)	83.40, 83.40, 83.40	28.23, 59.12, 45.42	142.71, 142.71, 83.13
α β γ °)	90, 90, 90	90, 106.73, 90	90, 90, 120
Resolution (Å)	24.07–2.60 (2.64–2.60)	43.31–1.60 (1.64–1.60)	32.79–3.17 (3.23–3.17)
Rsym or Rmerge	0.139 (0.655)	0.061 (0.123)	0.192 (0.777)
I/σI	46.2 (7.3)	17.1 (13.2)	18.1 (6.2)
Completeness (%)	98.6 (99)	99.9 (99.5)	92.2 (96.0)
Redundancy	37.0 (36.7)	3.7 (3.6)	58.8 (57.6)
Refinement
Resolution (Å)	24.07–2.60	43.41–1.60	32.79–3.17
No. reflections	3328	17905	8196
Rwork/Rfree	0.185/0.254	0.189/0.229	0.236/0.279
No. of atoms	657	1449	1865
Protein	624	1246	1850
Ligand/ion	N/A	N/A	N/A
Water	33	203	15
B-factors
Protein	42.58	5.158	93.63
Ligand/ion	N/A	N/A	N/A
Water	41.52	18.27	65.53
R.m.s. deviations
Bond lengths (Å)	0.002	0.023	0.003
Bond angles (°)	0.588	2.018	0.689
Ramachandran plot
Preferred regions (%)	94.3	94.9	88.5
Allowed regions (%)	5.7	4.4	6.6
Outliers (%)	0	0.7	4.9

Data for the highest resolution shell are shown in parentheses.