Figure 5.

c-kit^pos/CD34^neg/CD45^neg CPC numbers are increased in AMD3100-treated mice after ligation. CPCs were identified by staining for c-kit. Lineage of c-kit^pos CPCs was determined by counterstaining and colocalization with lymphoid, endothelial, and myeloid markers CD34 or CD45. (A) AMD3100-treatment increased c-kit^pos CPCs in the risk area of the LV after ligation.(B) CD34^pos and CD45^pos cells were present in the risk area but did not colocalize with c-kit^pos CPCs. (C) Representative confocal images of c-kit^pos, CD34^pos and CD45^pos cells in the risk area of AMD3100-treated ligated mice. Values are mean ± SEM (n=6). *P<0.05 vs Vehicle Ligation.