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. 2010 Jul 14;1(1):59–73. doi: 10.1364/BOE.1.000059

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©2010 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 1 — Schematic of the home-built multimodal nonlinear optical laser scanning microscope. Light source for TPEF and SHG imaging is a Ti:Sapphire femto-second oscillator (Tsunami,Spectra-Physics). For CARS imaging, Stokes pulses are generated in PCF which is pumped by the same Ti:sapphire laser. Inset shows a typical video image of a tissue sample with lumen surface facing up. F-ISO: Faraday isolator; CM: chirp laser mirrors; BS: beam splitter; NIR-F: near-IR filter;; GS: galvo scanner; DM: dichroic mirror; OBJ: objective lens; BF: bandpass filter; NDF: neutral density filter; λ/2: half waveplate.