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. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Biochemistry. 2010 Nov 29;49(50):10737–10746. doi: 10.1021/bi101014s

Figure 3.

Figure 3

GST pull-down assay showing the interaction between Ubp-M BUZ domain and histone (H3 + H4)2 tetramer. GST-BUZ (or GST) protein and (H3+H4)2 tetramer were sequentially loaded onto the column as input. The column was washed with 10 mL of PBS containing 0.1 M NaCl and then eluted with 3 × 200 μL of PBS containing 0.1 M NaCl (fractions 1–3), 3 × 200 μL of PBS containing 0.3 M NaCl (fractions 1–3), and 3 × 200 μL of PBS containing 0.7 M NaCl (fractions 1–3). Each fraction was analyzed by SDS-PAGE and stained by Coomassie blue. (a) Elution profile of the column loaded with GST-BUZ protein; (b) elution profile of the column loaded with GST alone. M, molecular weight markers.