. Author manuscript; available in PMC: 2011 Dec 21.

Published in final edited form as: Biochemistry. 2010 Nov 29;49(50):10737–10746. doi: 10.1021/bi101014s

Table 3.

Binding Affinity of Peptide Ligands to Ubp-M and HDAC6 BUZ Domains

Entry No.	Peptide Sequence^a	Peptide Source	K_D (μM)
Entry No.	Peptide Sequence^a	Peptide Source	Ubp-M BUZ^b	HDAC6 BUZ^c
1	IAKGG	library	12 ± 3	8.8 ± 1.1
2	NALGG	library	33 ± 7	0.79 ± 0.12
				0.23 ± 0.02 (non-GST)^d
3	LQDGF	library	>150	4.0 ± 1.3
				NA (non-GST) ^d
4	FDGFM	library	>150	10.8 ± 2.5
5	PSDHV	library	>140	ND
6	SLRAR	libraryc	ND	NA
7	RLRGG	ubiquitin	2.2 ± 0.4	0.34 ± 0.02
8	RLRGA		NA	NA
9	RLRAG		62 ± 11	NA
10	RLAGG		16 ± 4	0.90 ± 0.10
11	RARGG		2.0 ± 0.3	0.47 ± 0.02
12	ALRGG		2.3 ± 0.4	2.5 ± 0.4
13	STLGG	FBXO11	16 ± 4	3.7 ± 0.5
14	YGFGG	Histone H4	13 ± 3	6.2 ± 0.6
15	RGMGG	PTOV1	17 ± 3	1.3 ± 0.2
16	YUIGG	FAT10	12 ± 2	1.3 ± 0.1

Each peptide was labeled at the N-terminus with FITC via a β-Ala-β-Ala linker and contained a free C-terminus.

Ubp-M BUZ domain contained an N-terminal six-histidine tag.

GST-BUZ fusion protein was used in the binding studies unless otherwise noted.

Isolated BUZ domain (non-GST fusion) was used. NA, no significant binding affinity; ND, not determined; U, (S)-2-aminobutryic acid.