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. 2010 Dec 20;207(13):2869–2881. doi: 10.1084/jem.20100090

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Figure 4. — Productive HIV-1 infection of SEB-responding CD4 T cells is inhibited by in vitro neutralization of IL-2. (A) Productive HIV infection of CD4 T cells was analyzed after gating on CD4^low T cells and staining for HIV-p24 (y axis). Shown are dot plots from samples stimulated in the presence or absence of 1 µM AZT. (B) Dot plots from samples stimulated in the presence (bottom) or absence (top) of a neutralizing anti–IL-2 antibody comparing CFSE-fluorescence intensity (left), surface CD25 (middle), and CCR5 (right) with HIV-p24 staining. PBMC samples were stimulated for 24 h with SEB and then infected for another 24 h with the CCR5 tropic HIV-1 strain BAL.