Fig. 2.

Multiple sequence alignment of the amino acid sequences of the Nictaba domain from different plant species. Amino acids that were selected for mutation are indicated by the arrows. The Nictaba sequence is boxed. Accession numbers and species names are indicated in front of the sequence and after the sequence, respectively. Proteins that were aligned are, from top to bottom: hypothetical protein containing a TIR domain from Arabidopsis thaliana, dimeric phloem specific lectin PP2 from Cucurbita maxima, Nictaba from Nicotiana tabacum, F-box family protein from Populus trichocarpa, Putative F-box protein PP2-B12 from Arabidopsis thaliana, F-box family protein-like from Oryza sativa, F-box containing protein from Vitis vinifera, putative uncharacterized protein containing a F-Box domain from Populus trichocarpa, Phloem-specific lectin from Zea mays and predicted protein containing an F-box domain from Physcomitrella patens