Fig. 3.

Primary sequence of recombinant Nictaba, preceded by an N-terminal signal peptide necessary for secretion and a C-terminal tag containing of a c-myc epitope and a (His)6 tag (a). Proteins were analyzed by SDS-PAGE (b) and Western blot analysis with a monoclonal anti-His antibody (c). Samples are loaded as follows: lane 1: molecular weight marker, lane 2: native Nictaba from tobacco; lane 3: recombinant Nictaba; lane 4: Nictaba mutant 1; lane 5: Nictaba mutant 2; lane 6: Nictaba mutant 3. Approximately 0.5 μg of each protein was loaded on the gel