Table 1.

List of primer sequences used to amplify the original and mutant forms of Nictaba

Primer	Properties	Sequence (5′-3′)
Native Nictaba
Evd 65	Forward	cag tgg ata gcc gca aga gac ctt tc
Evd 66	Reverse	tta gtt tgg acg aat gtc gaa gcc c
Nictaba mutant 1
Evd 12	5′ EcoRI	ggc gga gaa ttc acc atg caa ggc cag tgg ata gcc gc
Evd 303	Reverse: E128→A128/E135→A135	att gat tgc cat caa cct cat ttc gac tgc acc atc ctc
Evd 302	Forward: E128→A128;E135→A135	aga gga tgg tgc agt cga aat gag gtt gat ggc aat caa t
Evd 315	3′ XbaI	ccc gct ttc tag aca gtt tgg acg aat gtc gaa gcc
Nictaba mutant 2/3
Evd 376	5′ EcoRI	gaa ttc acc atg caa ggc cag tgg ata
Evd 377	Reverse
	Mutant 2: W22→L22;W15→L15	tgt caa gta ctg agg att gtc cac caa tgt
	Mutant 3: W15→L15	tgt caa gta ctg agg att gtc cac cca tgt
Evd 378	Forward
	Mutant 2: W15→L15;W22→L22	aca ttg gtg gac aat cct cag tac ttg aca
	Mutant 3: W15→L15	aca ttg gtg gac aat cct cag tac tgg aca
Evd 379	3′ XbaI	tct aga cag ttt gga cga atg tcg aag