Table 2.
Comparative analysis of glycan array results for native and recombinant Nictaba as well as Nictaba mutants 1 and 3
| Native Nictaba |
Recombinant Nictaba |
Nictaba mutant 1 v3.0 |
Nictaba mutant 3 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Chart number v4.0 | Structure | RFU | Ranka | RFU | Ranka | RFU | Ranka | RFU | Ranka |
| NA | Galβ1-4GlcNAcβ1-2Manα1-3(Fucα1-3(Galβ1-4)GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | NAb | NA | NA | NA | 52664 | 100 | NA | NA |
| 202 | Manα1-2Manα1-6(Manα1-3)Manα1-6[Manα1-2Manα1-2Manα1-3]Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 32124 | 100 | 4775 | 46 | 19894 | 38 | 1 | 0 |
| 201 | Manα1-6[Manα1-2Manα1-3]Manα1-6[Manα1-2Manα1-3] Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 30941 | 96 | 2651 | 26 | 27651 | 53 | 6 | 0 |
| 368 | Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ1-2Manα1-3(Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | 26138 | 81 | 5182 | 50 | NA | NA | 1 | 0 |
| 322 | Fucα1-3(Galβ1-4)GlcNAcβ1-2Manα1-3(Fucα1-3(Galβ1-4) GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | 25973 | 81 | 10296 | 100 | 36925 | 70 | −2 | 0 |
| 346 | Galβ1-4GlcNAcβ1-2Manα1-3(Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 25012 | 78 | 3395 | 33 | NA | NA | 3 | 0 |
| 206 | Manα1-6(Manα1-3)Manα1-6[Manα1-2Manα1-3] Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 23426 | 73 | 2321 | 23 | 29479 | 56 | 1 | 0 |
| 48 | Manα1-3(Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp13 | 19194 | 60 | 1394 | 14 | 29183 | 55 | 12 | 0 |
| 207 | Manα1-6(Manα1-3)Manα1-6(Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 18865 | 59 | 1616 | 16 | 47537 | 90 | 7 | 0 |
| 47 | Manα1-3(Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 18598 | 58 | 913 | 9 | NA | NA | 1 | 0 |
| 319 | Galβ1-3GlcNAcβ1-2Manα1-3(Galβ1-3GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp19 | 16215 | 50 | 360 | 3 | 124 | 0 | 2 | 0 |
| 301 | GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 15637 | 49 | 273 | 3 | 2015 | 4 | 2 | 0 |
| 359 | Galα1-3Galβ1-4GlcNAcβ1-2Manα1-3(Galα1-3Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | 15277 | 48 | 334 | 3 | NA | NA | 6 | 0 |
| 344 | Galβ1-4GlcNAcβ1-2Manα1-3Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 14927 | 46 | 1188 | 12 | NA | NA | 3 | 0 |
| 203 | Manα1-2Manα1-2Manα1-3(Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 14796 | 46 | 1605 | 16 | 9830 | 19 | 2 | 0 |
| 357 | Fucα1-2Galβ1-4GlcNAcβ1-2Manα1-3(Fucα1-2Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | 14054 | 44 | 169 | 2 | NA | NA | 4 | 0 |
| 367 | Galα1-3(Fucα1-2)Galβ1-4GlcNAcβ1-2Manα1-3(Galα1-3(Fucα1-2)Galβ1-4GlcNAcβ1-2Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp20 | 13121 | 41 | 168 | 2 | NA | NA | 7 | 0 |
| 348 | Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAcβ-Sp22 | 12978 | 40 | 352 | 3 | NA | NA | 6 | 0 |
| 180 | (GlcNAcβ1-4)5β-Sp8 | 12695 | 40 | 446 | 4 | 6910 | 13 | 2 | 0 |
| 349 | Galβ1-3GlcNAcβ1-2Manα1-3(Galβ1-3GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAcβ-Sp22 | 12408 | 39 | 115 | 1 | NA | NA | 4 | 0 |
| 393 | Galβ1-4GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAc-Sp12 | 11677 | 36 | 331 | 3 | NA | NA | −1 | 0 |
| 179 | (GlcNAcβ1-4)6β-Sp8 | 11325 | 35 | 439 | 4 | 3909 | 7 | 5 | 0 |
| 50 | Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-Sp12 | 10836 | 34 | 163 | 2 | 2585 | 5 | 4 | 0 |
| 442 | Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-2(Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-4)Manα1-3(Fucα1-2Galβ1-4 (Fucα1-3)GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAcβ-N | 10726 | 33 | 2006 | 19 | NA | NA | 1 | 0 |
| 358 | Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-2Manα1-3(Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1-2Manα1-6) Manβ1-4GlcNAcβ1-4GlcNAβ-Sp20 | 10151 | 32 | 2110 | 20 | NA | NA | 1 | 0 |
a
Percentile ranking: The glycan with the highest RFU is assigned a value of 100
b
NA means that the glycan was not available in that assay; i.e., present on version 4 but not version 3, or present on version 3 and not on version 4