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. Author manuscript; available in PMC: 2010 Dec 21.
Published in final edited form as: In Vitro Cell Dev Biol. 1988 May;24(5):464–470. doi: 10.1007/BF02628499

FIG. 2.

FIG. 2

Phase contrast microscopy of the cell cultures. a, Initial isolates consist largely of “organoid” clusters of cells (inset) which after 24 h begin to spread over the plate. b, After 8 to 12 d the plates become confluent with a cobblestone appearance. c, The cells begin to form spheres containing debris after 3 to 4 wk (bottom arrow). Note the smaller polygonal cells in the top center of c and the nearby multinucleated cell (top arrow). Immunoperoxidase staining for prekeratin reveals that virtually all cells are positive (d) showing crisscrossing fibrils in the cytoplasm (inset).