Table 1.
Quantitation of connective tissue mineralization of vibrissae*.
| Mouse | Diet | Diet started → diet end (months) | No. of mice | Mineralized vibrissae (per mouse) | Total vibrissae examined (per mouse) | Percent mineralized† | Area of mineralization per total area of vibrissae (%)† | Fold‡ | p‐value |
|---|---|---|---|---|---|---|---|---|---|
| Wild‐type | Control | 1 → 6 | 9 | 0 | 11.0 | 0 | 0 | ||
| Abcc6−/− | Control | 1 → 3 | 8 | 5.5 | 10.3 | 58.1 ± 5.5 | 0.69 ± 0.14 | 1.0 | 0.001§ |
| Abcc6−/− | Control | 1 → 6 | 9 | 6.4 | 8.8 | 75.2 ± 5.2 | 2.78 ± 0.76 | 4.0 | 0.001§ |
| Abcc6−/− | Magnesium carbonate | 1 → 3 | 6 | 0 | 7.7 | 0 | 0 | 0 | 0.001∥ |
| Abcc6−/− | Magnesium carbonate | 1 → 6 | 5 | 0.2 | 6.8 | 4.0 ± 4.0 | 0.15 ± 0.15 | 0.2 | 0.003∥ |
| Abcc6−/− | Magnesium carbonate | 3 → 6 | 8 | 4.6 | 8.4 | 49.3 ± 12.4 | 0.97 ± 0.33 | 1.4 | 0.074∥,¶ |
| Abcc6−/− | Lanthanum carbonate | 1 → 3 | 6 | 2.5 | 13.2 | 23.3 ± 15.9 | 0.72 ± 0.39 | 1.0 | 0.555∥ |
| Abcc6−/− | Lanthanum carbonate | 1 → 6 | 5 | 5.6 | 6.0 | 92.5 ± 5.0 | 1.92 ± 0.28 | 2.8 | 0.884∥ |
| Abcc6−/− | Lanthanum carbonate | 3 → 6 | 5 | 5.0 | 8.5 | 60.0 ± 5.7 | 2.32 ± 0.49 | 3.4 | 0.897∥ |
* Skin sections containing vibrissae were stained with H&E and examined by computerized morphometric analysis.
† Values presented as mean ± S.E.
‡ Calculated using the 3‐month‐old Abcc6 −/− mice as 1.0.
§ Compared with the wild type mice.
∥ Compared with age‐matched Abcc6 −/− mice on control diet.
¶ Statistical difference by Student's t‐test, p= 0.0497.