Table 1. Expected and observed monoisotopic masses (MH+) of tryptic fragments from NEM-reacted mitochondrial Trx 2.
Fragment | Expected mass (MH+) | Observed mass (MH+) |
---|---|---|
Cys-containing peptides | ||
T2 dithiols (Cys 90, Cys93) | 2315.09 | 2315.15 |
T2-1NEM | 2440.09 | 2440.2 |
T2-2NEM | 2565.09 | ND |
| ||
Other identified peptides | ||
T1 | 1653.75 | 1653.80 |
T8 | 2744.36 | 2745.45 |
| ||
Amino acid sequences of the Trx2 tryptic fragments | ||
60 TTFNIQDGPDFQDR(T1), VVNSET PVVVDFHAQWCGPCK(T2), 95 ILGPR(T3), LEK(T4), MVAK(T5), QHG K(T6),VVMAK(T7), 116 VDIDDHTDLAIEYEVSAVPTVLAMK(T8), NGDVVDK(T9), 149 FVGIK(T10), DEDQLEAFLKK(T11), LIG(T12) | ||
The mitochondrial localization signal from 1 to 59, which is removed upon mitochondrial import, is not shown. |