Table 2. Effect of Preincubation of PBL With FK on the Cellular Content of the Drug.
Subject | FK (ng/106 Cells) | |
---|---|---|
Control* | Experimental* | |
1 | a. 0.05 | 0.07 |
b. 0.12 | 0.25 | |
2 | a. 0.19 | 0.38 |
b. 0.20 | 0.79 | |
3 | a. 0.49 | 0.69 |
b. 1.58 | Not measured |
PBL were preincubated for 24 hours in 1% BSA with and without (control) 1 μg FK/mL medium.
After preincubation and washings, control and experimental cells were incubated with 1 and 4 × 10−7 mol/L, a and b respectively, concentrations of FK for one hour. The FK content in the cells was measured by enzyme-linked immunosorbent assay.