FIG. 6.
A group II mGluR antagonist increases excitability of layer V pyramidal cells. A: LY341495 increases numbers of action potentials evoked by depolarizing current pulses. Current-clamp recording of responses to increasingly intense depolarizing current pulses before (darker trace, control) and after local perfusion of LY341495 (lighter trace). Vm: −61 mV in control and −59 mV after LY341495. Spikes are truncated. Spike height: 64 mV. B: graph of the average number of spikes evoked by current pulses with amplitudes shown in A. Data show means for 6 cells under control (Pre-Drug) and LY341495 conditions. Fifteen to 20 consecutive responses were used to generate the average for each cell, in each condition. *, P < 0.05, Pre-Drug vs. LY341495. C: graph of the membrane potential before, during, and after local perfusion of LY341495 (2 μM; n = 6; horizontal bar).