Table 1. Effect of OH-F prepared from different sources on DNA synthesis, percentage of labeled nuclei, and percentage of mitosis in 40% hepatectomized rats.
The preparation of ethanol precipitate fraction (OH-F), determination of [3H] thymidine incorporation, labeling index, and mitotic index have been described in “Materials and Methods.” Rats received 10 mg of OH-F i.p. in 2 ml 5 mm phosphate buffer.
| Source of OH-F | No. of rats | [3H]thymidine incorporation (cpm/mg DNA) | % of labeled nuclei | % of mitosis |
|---|---|---|---|---|
| Sham-operated rat liver (control) | 8 | 13,130 ± 690a | 12.8 ± 1.8 | 0.7 ± 0.1 |
| 70% PH rat liver | 14 | 63,520 ± 18,100b | ||
| Weanling rat liver | 16 | 60,750 ± 13,320b | 25.8 ± 5b | 1.8 ± 0.2b |
a
Mean ± SD.
b
Significantly different from sham-operated rat liver (control value); P< 0.05.