Target molecules are present on the cell surface of primary AML LSCs. MFI of expression for CD32, CD25, CD18, and CD93 in 61 primary AML patient BM LSCs with normal human BM HSCs as controls. Cutoff MFI = 800 (indicated as a horizontal line). Red, black, and blue circles represent marker-positive AML LSCs, marker-negative AML LSCs, and normal BM HSCs, respectively. AML: M0, n = 2; M1, n = 8; M2, n = 15; M4, n = 4; other AML (M5, M7, and undetermined), n = 1 each; MDS/AML: n = 29; normal human BM: n = 5.