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. 2010 Oct;13(5):527–537. doi: 10.1089/rej.2009.1006

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 1. — Ibuprofen treatment induces p75^NTR expression and reduces the survival of U87MG and CCFSTTG1 cells in a time-dependent manner. (A) Representative Western blot of p75^NTR levels in CCFSTTG1 and U87MG cells treated with 0 or 2 mM ibuprofen for 24 and 48 h. Cells were lysed, and equal amounts of protein (40 μg) were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Immunoblots were stripped and reprobed for β-actin to ensure equal loading, and the A875 melanoma lysates were used as a positive control for p75^NTR. (B) Relative survival of CCFSTTG1 and U87MG cells after ibuprofen treatment for 24 or 48 h; data are presented as mean cell survival ± standard error of the mean (SEM), normalized to levels in vehicle-treated (dimethylsulfoxide [DMSO]) cells with ** indicating p < 0.01, as compared to vehicle-treated cells.

FIG. 1. — Ibuprofen treatment induces p75^NTR expression and reduces the survival of U87MG and CCFSTTG1 cells in a time-dependent manner. (A) Representative Western blot of p75^NTR levels in CCFSTTG1 and U87MG cells treated with 0 or 2 mM ibuprofen for 24 and 48 h. Cells were lysed, and equal amounts of protein (40 μg) were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Immunoblots were stripped and reprobed for β-actin to ensure equal loading, and the A875 melanoma lysates were used as a positive control for p75^NTR. (B) Relative survival of CCFSTTG1 and U87MG cells after ibuprofen treatment for 24 or 48 h; data are presented as mean cell survival ± standard error of the mean (SEM), normalized to levels in vehicle-treated (dimethylsulfoxide [DMSO]) cells with ** indicating p < 0.01, as compared to vehicle-treated cells.