FIG. 4.
Evaluation of biochanin A (BCA) effect in the presence of p75NTR siRNA. MTT analysis of U87 (A, left) and CCFSTTG1 (A, right) cells transfected with 100 nM scrambled (sc) or p75NTR siRNA, followed by treatment with ibuprofen (0 or 2 mM) after 24 h for 48 h (intervention regimen) or vehicle (dimethylsulfoxide [DMSO]) for 24 h and then ibuprofen for 24 h (prevention regimen). (B) U87 cells treated with scrambled (sc) or p75NTR siRNA for 24 h followed by ibuprofen (0 or 2 mM) for 24 h and then ibuprofen + BCA (0, 10, 20, 40, 60, 80, 100 μM) for an additional 24 h (left) or BCA (0, 10, 20, 40, 60, 80, 100 μM) for 24 h followed then by ibuprofen + BCA (0, 10, 20, 40, 60, 80, 100 μM) for an additional 24 h (right). (C) CCFSTTG1 cells treated with scrambled (sc) or p75NTR siRNA for 24 h followed by ibuprofen (2 mM) for 24 h and then ibuprofen + BCA (0, 10, 20, 40, 60, 80, 100 μM) for an additional 24 h (left) or BCA (0, 10, 20, 40, 60, 80, 100 μM) for 24 h followed by then ibuprofen + BCA (0, 10, 20, 40, 60, 80, 100 μM) for an additional 24 h (right). Data are presented as mean cell survival ± SEM, normalized to vehicle-treated cells only, with NS, *, **, and *** indicating nonsignificant, p < 0.05, 0.01, and 0.001, respectively, as compared to ibuprofen only-treated cells.