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. Author manuscript; available in PMC: 2012 Jan 1.

Published in final edited form as: J Surg Res. 2010 Oct 13;165(1):5–14. doi: 10.1016/j.jss.2010.09.018

4A. Effect of increasing concentration of malonate on mitochondrial hydrogen peroxide production. At different respiratory rates, IPC mitochondria produced less H₂O₂. IPC had a lower basal rate of H₂O₂ production (omM malonate point). Inhibition of respiration decreased ROS production in both groups, with IPC producing less at all points. *p<0.05 vs. Control IR, n=6/group.

4B. H₂O₂ production as measured by fluorometry. In the presence of horseradish peroxidase (catalyst), Amplex-red reacts with H₂O₂ to produce the highly fluorescent compound resorufin. Catalase reduces H₂O₂ to H₂O and was used as a negative control. The addition of catalase to the reaction chamber abolishes any increase in fluorometric signal.

4A. Effect of increasing concentration of malonate on mitochondrial hydrogen peroxide production. At different respiratory rates, IPC mitochondria produced less H₂O₂. IPC had a lower basal rate of H₂O₂ production (omM malonate point). Inhibition of respiration decreased ROS production in both groups, with IPC producing less at all points. *p<0.05 vs. Control IR, n=6/group.

4B. H₂O₂ production as measured by fluorometry. In the presence of horseradish peroxidase (catalyst), Amplex-red reacts with H₂O₂ to produce the highly fluorescent compound resorufin. Catalase reduces H₂O₂ to H₂O and was used as a negative control. The addition of catalase to the reaction chamber abolishes any increase in fluorometric signal.