Fig 7.

SRp20 regulates expression of FoxM1, Cdc25B, and PLK1 and is essential for cell cycle progression. (A) U2OS and HeLa cells with SRp20 knocked down by RNAi were collected for flow cytometry. NS, non-specific siRNA. One representative of two separate experiments is shown. (B and C) SRp20 regulates expression of FoxM1, Cdc25B, and PLK1. U2OS and HeLa cells with SRp20 knockdown as described in Fig. 3 were examined for FoxM1, Cdc25B, and PLK1 expression at the RNA level by RT-PCR (B) and at the protein level by immunoblot (C). Diagrams to the right of B show RNA splicing directions of FoxM1, Cdc25B, and PLK1 and primer positions (short lines above or below exons [boxes]) for RT-PCR; relative expression levels of each RNA transcript after being normalized to GAPDH are indicated underneath the gels. RT, reverse transcription. Protein levels of SRp20, FoxM1, Cdc25B, and PLK1 were also measured by Western blot (C); GAPDH (B) and hnRNP K (C) served as controls for sample loading in each assay.