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Stable expression of T7-SRp20 in MEF 3T3 tet-off cells promotes cell cycle progression. MEF 3T3 tet-off cells stably transfected with a T7-SRp20 vector or an empty vector were grown in doxycycline-free medium, synchronized by starvation for 48 h, and collected after the indicated durations of serum stimulation for cell cycle flow cytometry. One representative of two separate experiments is shown.
