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Expression of Sox21 in mouse ESCs undergoing differentiation in response to elevated levels of Sox2. qPCR was performed on cDNA prepared from RNA isolated from untreated i-Sox2-ESCs or i-Sox2-ESCs treated with 4 μg/ml Dox for 9, 24, or 72 h, as described in Materials and Methods. Results are presented as fold-enrichment relative to GAPDH in Dox-induced cells vs. uninduced cells.
