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. 2011 Jan;25(1):219–231. doi: 10.1096/fj.10-167361

Figure 3.

Figure 3.

RSVA314 and RSVA405 are potent activators of AMPK. A–D) APP-HEK293 cells were treated for 24 h with DMSO (control, A), RSVA314 (3 μM, B), or RSVA405 (3 μM, C). Cell extracts were then probed on human phosphoprotein arrays. Representative phospho-protein array analyses are shown in A–C. Boxes 1–3 in A–C indicate phospho-Thr-172 AMPKα2, phospho-Ser-133 CREB, and phospho-Thr-68 Chk2, respectively. Results are expressed as percentage of the control levels (D). E–G) APP-HEK293 (E), APP-SY5Y (F), and wild-type HEK293 cells (G) were treated for 24 h with the indicated concentrations of RSVA314 and RSVA405. Cell extracts were then analyzed by WB for secreted Aβ or cellular phospho-Thr-172 AMPK (pAMPK), AMPK, phospho-Ser-79 ACC (pACC), ACC, phospho-Ser-133 CREB (pCREB), CREB, phospho-Ser-63 c-Jun (pc-Jun), c-Jun, c-Fos, or actin levels.