Figure 5.

Effect of RSVA314 and RSVA405 on Aβ levels is inhibited in cells expressing inactive AMPK. APP-HEK293 cells were transfected with control vector (V) and Myc-tagged dominant negative T172A-AMPK (T172A, A–F) or Myc-tagged kinase-dead K45R-AMPK (K45R, G–L). At 24 h post-transfection, cells were treated for 24 h with DMSO (CTRL) or 3 μM RSVA314 (A–C, G-I) or 3 μM RSVA405 (D–F, J–L). Myc-AMPK, pACC, ACC, pRaptor, actin, or secreted Aβ levels were analyzed by WB (A, D, G, J). B, E, H, K) Densitometric analyses and quantification of the ratio pACC/ACC from experiments as in A, D, G, J. C, F, I, L) ELISA measurements of secreted Aβ. Histograms show means ± sd of 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's t test).