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. 2011 Jan;25(1):255–264. doi: 10.1096/fj.10-155291

Figure 4.

Figure 4.

Gap junction inhibitors carbenoxolone (CBX) and 1-heptanol inhibit microtissue self-assembly and self-sorting. A) Carbenoxolone inhibited KGN rod self-assembly in a dose-dependent manner, with increased inhibition on pretreatment. B) 1-Heptanol also inhibited self-assembly in a dose-dependent manner. C) Live/dead analysis confirms that microtissue viability was not compromised, n = 35. D) Percentage inhibition of rod contraction by carbenoxolone varied with postseeding time and cell type; n = 18 (KGN), 11 (MCF-7), and 19 (NHF). KGN cells labeled with CellTracker Green (CMFDA) were mixed with HeLa cells labeled with CellTracker Blue (CMAC) at a 1:1 ratio and seeded into spheroid gels. E) Cells were clearly segregated by 16 h postseeding, with the connexin-expressing cells (KGN) sorting to the center and the connexin-negative cells (HeLa) sorting to the periphery. Treatment with carbenoxolone (50 μM) disrupted sorting, with an increased degree of mixing. EF, epifluorescence; CF, confocal. Scale bar = 200 μm.